Detection and genetic characterization of foot-and-mouth disease viruses in samples from clinically healthy animals in endemic settings
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Detection and genetic characterization of foot-and-mouth disease viruses in samples from clinically healthy animals in endemic settings. / Jamal, S M; Ferrari, G; Hussain, M; Nawroz, A H; Aslami, A A; Khan, E; Murvatulloev, S; Ahmed, S; Belsham, G J.
I: Transboundary and Emerging Diseases, Bind 59, Nr. 5, 10.2012, s. 429-40.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Detection and genetic characterization of foot-and-mouth disease viruses in samples from clinically healthy animals in endemic settings
AU - Jamal, S M
AU - Ferrari, G
AU - Hussain, M
AU - Nawroz, A H
AU - Aslami, A A
AU - Khan, E
AU - Murvatulloev, S
AU - Ahmed, S
AU - Belsham, G J
N1 - © 2011 Blackwell Verlag GmbH.
PY - 2012/10
Y1 - 2012/10
N2 - A total of 1501 oral swab samples from Pakistan, Afghanistan and Tajikistan were collected from clinically healthy animals between July 2008 and August 2009 and assayed for the presence of foot-and-mouth disease virus (FMDV) RNA. The oral swab samples from two (of four) live animal markets in Pakistan (n = 245), one (of three) live animal market in Afghanistan (n=61) and both the live animal markets in Tajikistan (n=120) all tested negative. However, 2 of 129 (∼2%) samples from Gondal and 11 of 123 (9%) from Chichawatni markets in Pakistan were positive for FMDV RNA. Similarly, 12 of 81 (15%) samples from Kabul and 10 of 20 (50%) from Badakhshan in Afghanistan were found to be positive. Serotypes A and O of FMDV were identified within these samples. Oral swab samples were also collected from dairy colonies in Harbanspura, Lahore (n=232) and Nagori, Karachi (n=136), but all tested negative for FMDV. In the Landhi dairy colony, Pakistan, a cohort of 179 apparently healthy animals was studied. On their arrival within the colony, thirty-nine (22%) of these animals were found positive for FMDV RNA (serotype A was identified), while 130 (72.6%) had antibodies to FMDV non-structural proteins. Thus, newly introduced animals may be a significant source of the disease in the colony. Only two animals from the cohort were detected as becoming positive for FMDV RNA during a follow-up period of 4months; however, only 10 animals remained negative for anti-NSP antibodies during this period.
AB - A total of 1501 oral swab samples from Pakistan, Afghanistan and Tajikistan were collected from clinically healthy animals between July 2008 and August 2009 and assayed for the presence of foot-and-mouth disease virus (FMDV) RNA. The oral swab samples from two (of four) live animal markets in Pakistan (n = 245), one (of three) live animal market in Afghanistan (n=61) and both the live animal markets in Tajikistan (n=120) all tested negative. However, 2 of 129 (∼2%) samples from Gondal and 11 of 123 (9%) from Chichawatni markets in Pakistan were positive for FMDV RNA. Similarly, 12 of 81 (15%) samples from Kabul and 10 of 20 (50%) from Badakhshan in Afghanistan were found to be positive. Serotypes A and O of FMDV were identified within these samples. Oral swab samples were also collected from dairy colonies in Harbanspura, Lahore (n=232) and Nagori, Karachi (n=136), but all tested negative for FMDV. In the Landhi dairy colony, Pakistan, a cohort of 179 apparently healthy animals was studied. On their arrival within the colony, thirty-nine (22%) of these animals were found positive for FMDV RNA (serotype A was identified), while 130 (72.6%) had antibodies to FMDV non-structural proteins. Thus, newly introduced animals may be a significant source of the disease in the colony. Only two animals from the cohort were detected as becoming positive for FMDV RNA during a follow-up period of 4months; however, only 10 animals remained negative for anti-NSP antibodies during this period.
KW - Afghanistan/epidemiology
KW - Animals
KW - Buffaloes
KW - Cattle
KW - Dairying
KW - Endemic Diseases/veterinary
KW - Foot-and-Mouth Disease/epidemiology
KW - Foot-and-Mouth Disease Virus/genetics
KW - Genetic Variation
KW - Logistic Models
KW - Multivariate Analysis
KW - Pakistan/epidemiology
KW - Phylogeny
KW - RNA, Viral/isolation & purification
KW - Real-Time Polymerase Chain Reaction
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Tajikistan/epidemiology
U2 - 10.1111/j.1865-1682.2011.01295.x
DO - 10.1111/j.1865-1682.2011.01295.x
M3 - Journal article
C2 - 22212855
VL - 59
SP - 429
EP - 440
JO - Transboundary and Emerging Diseases
JF - Transboundary and Emerging Diseases
SN - 1865-1674
IS - 5
ER -
ID: 257917246