Protein phosphatase 2A isotypes regulate cell surface expression of the T cell receptor

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Standard

Protein phosphatase 2A isotypes regulate cell surface expression of the T cell receptor. / Lauritsen, Jens Peter Holst; Menné, C; Kastrup, J; Dietrich, J; Geisler, C.

I: Experimental and Clinical Immunogenetics, Bind 18, Nr. 1, 2001, s. 24-33.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Lauritsen, JPH, Menné, C, Kastrup, J, Dietrich, J & Geisler, C 2001, 'Protein phosphatase 2A isotypes regulate cell surface expression of the T cell receptor', Experimental and Clinical Immunogenetics, bind 18, nr. 1, s. 24-33.

APA

Lauritsen, J. P. H., Menné, C., Kastrup, J., Dietrich, J., & Geisler, C. (2001). Protein phosphatase 2A isotypes regulate cell surface expression of the T cell receptor. Experimental and Clinical Immunogenetics, 18(1), 24-33.

Vancouver

Lauritsen JPH, Menné C, Kastrup J, Dietrich J, Geisler C. Protein phosphatase 2A isotypes regulate cell surface expression of the T cell receptor. Experimental and Clinical Immunogenetics. 2001;18(1):24-33.

Author

Lauritsen, Jens Peter Holst ; Menné, C ; Kastrup, J ; Dietrich, J ; Geisler, C. / Protein phosphatase 2A isotypes regulate cell surface expression of the T cell receptor. I: Experimental and Clinical Immunogenetics. 2001 ; Bind 18, Nr. 1. s. 24-33.

Bibtex

@article{948c0350b0a111ddb538000ea68e967b,
title = "Protein phosphatase 2A isotypes regulate cell surface expression of the T cell receptor",
abstract = "The mechanisms underlying T cell receptor (TCR) down-regulation have been extensively studied during the last decade. Whereas the importance of phosphorylation in this process has been established, it is less certain whether dephosphorylation plays a role in TCR down-regulation. In this study, we show that inhibition of the serine/threonine protein phosphatase PP2A family had a biphasic effect on TCR expression. Thus, low concentrations of PP2A inhibitors induced TCR down-regulation, whereas higher concentrations of PP2A inhibitors induced TCR up-regulation. The effect of PP2A inhibition was independent of phosphorylation of the CD3gamma endocytosis motif. Whereas TCR down-regulation was caused by a partial inhibition of exocytosis, TCR up-regulation was caused by an inhibition of endocytosis. The effects on exocytosis and endocytosis were not restricted to the TCR, indicating a more general regulatory role for PP2A in both exocytosis and endocytosis.",
author = "Lauritsen, {Jens Peter Holst} and C Menn{\'e} and J Kastrup and J Dietrich and C Geisler",
note = "Keywords: Amino Acid Sequence; Antibiotics, Antifungal; Antigens, CD3; Down-Regulation; Endocytosis; Enzyme Inhibitors; Exocytosis; Humans; Isoenzymes; Jurkat Cells; Molecular Sequence Data; Okadaic Acid; Oxazoles; Phosphoprotein Phosphatases; Phosphorylation; Protein Phosphatase 2; Pyrans; Receptors, Antigen, T-Cell; Receptors, Antigen, T-Cell, gamma-delta; Receptors, Transferrin; Spiro Compounds",
year = "2001",
language = "English",
volume = "18",
pages = "24--33",
journal = "Experimental and Clinical Immunogenetics",
issn = "0254-9670",
publisher = "S Karger AG",
number = "1",

}

RIS

TY - JOUR

T1 - Protein phosphatase 2A isotypes regulate cell surface expression of the T cell receptor

AU - Lauritsen, Jens Peter Holst

AU - Menné, C

AU - Kastrup, J

AU - Dietrich, J

AU - Geisler, C

N1 - Keywords: Amino Acid Sequence; Antibiotics, Antifungal; Antigens, CD3; Down-Regulation; Endocytosis; Enzyme Inhibitors; Exocytosis; Humans; Isoenzymes; Jurkat Cells; Molecular Sequence Data; Okadaic Acid; Oxazoles; Phosphoprotein Phosphatases; Phosphorylation; Protein Phosphatase 2; Pyrans; Receptors, Antigen, T-Cell; Receptors, Antigen, T-Cell, gamma-delta; Receptors, Transferrin; Spiro Compounds

PY - 2001

Y1 - 2001

N2 - The mechanisms underlying T cell receptor (TCR) down-regulation have been extensively studied during the last decade. Whereas the importance of phosphorylation in this process has been established, it is less certain whether dephosphorylation plays a role in TCR down-regulation. In this study, we show that inhibition of the serine/threonine protein phosphatase PP2A family had a biphasic effect on TCR expression. Thus, low concentrations of PP2A inhibitors induced TCR down-regulation, whereas higher concentrations of PP2A inhibitors induced TCR up-regulation. The effect of PP2A inhibition was independent of phosphorylation of the CD3gamma endocytosis motif. Whereas TCR down-regulation was caused by a partial inhibition of exocytosis, TCR up-regulation was caused by an inhibition of endocytosis. The effects on exocytosis and endocytosis were not restricted to the TCR, indicating a more general regulatory role for PP2A in both exocytosis and endocytosis.

AB - The mechanisms underlying T cell receptor (TCR) down-regulation have been extensively studied during the last decade. Whereas the importance of phosphorylation in this process has been established, it is less certain whether dephosphorylation plays a role in TCR down-regulation. In this study, we show that inhibition of the serine/threonine protein phosphatase PP2A family had a biphasic effect on TCR expression. Thus, low concentrations of PP2A inhibitors induced TCR down-regulation, whereas higher concentrations of PP2A inhibitors induced TCR up-regulation. The effect of PP2A inhibition was independent of phosphorylation of the CD3gamma endocytosis motif. Whereas TCR down-regulation was caused by a partial inhibition of exocytosis, TCR up-regulation was caused by an inhibition of endocytosis. The effects on exocytosis and endocytosis were not restricted to the TCR, indicating a more general regulatory role for PP2A in both exocytosis and endocytosis.

M3 - Journal article

C2 - 11150850

VL - 18

SP - 24

EP - 33

JO - Experimental and Clinical Immunogenetics

JF - Experimental and Clinical Immunogenetics

SN - 0254-9670

IS - 1

ER -

ID: 8544877